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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
04/03/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MENA, E.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
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Afiliación : |
EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCOS MONTESANO, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Soybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733 |
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DOI : |
10.3389/fpls.2019.01733 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020.
The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826). |
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Contenido : |
Abstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses. MenosAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xyl... Presentar Todo |
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Palabras claves : |
CELL WALL; DEFENSE GENES; DIAPORTHE CAULIVORA; DISEASE SYMPTOMS; INTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA); PATHOGEN COLONIZATION; SOYBEAN STEM CANKER; TRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a). |
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Thesagro : |
ENFERMEDADES DE LAS PLANTAS; PATÓGENOS; SOJA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16699/1/fpls-10-01733.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2019.01733/pdf
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Marc : |
LEADER 03822naa a2200313 a 4500
001 1060886
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2019.01733$2DOI
100 1 $aMENA, E.
245 $aSoybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020. The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826).
520 $aAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses.
650 $aENFERMEDADES DE LAS PLANTAS
650 $aPATÓGENOS
650 $aSOJA
653 $aCELL WALL
653 $aDEFENSE GENES
653 $aDIAPORTHE CAULIVORA
653 $aDISEASE SYMPTOMS
653 $aINTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA)
653 $aPATHOGEN COLONIZATION
653 $aSOYBEAN STEM CANKER
653 $aTRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a)
700 1 $aSTEWART, S.
700 1 $aMONTESANO, M.
700 1 $aPONCE DE LEÓN, I.
773 $tFrontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/07/2023 |
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Actualizado : |
24/07/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
CAFFARENA, D.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Uso de prácticas de manejo del dolor durante el desbotonamiento y descorne de las terneras de tambo: un estudio piloto en Uruguay y Argentina. [Use of pain management procedures during disbudding/dehorning of dairy heifers: a pilot study in Uruguay and Argentina.] |
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Complemento del título : |
Sección: Artículos originales. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Veterinaria (Montevideo), 2018, v. 54, no. 210, p. 22-26. -- OPEN ACCESS. |
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ISSN : |
0376-4362 (impresa); 1688-4809 (en línea). |
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DOI : |
10.29155/VET.54.210.4 |
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Idioma : |
Español |
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Notas : |
Article history: Recibido 20 Diciembre 2017; Aceptado 29 Mayo 2018; Publicado 1 Noviembre 2018. -- Autor para correspondencia: dcaffarena@inia.org.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU). |
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Contenido : |
RESUMEN.- El desbotonamiento/descorne en los bovinos lecheros es una práctica muy difundida que facilita el manejo, aunque provoca
estrés y dolor. Para evaluar preliminarmente qué tan difundida es la aplicación de procedimientos de manejo del dolor (analgesia/anestesia) durante esta maniobra entre veterinarios asesores de tambos de Uruguay y Argentina, se realizó un cuestionario distribuido por correo electrónico a 120 veterinarios. Treinta y seis de los 38 veterinarios (94,7%) que respondieron a la encuesta realizaba este procedimiento en los tambos que asesoraba. De estos 36, el 75% (27) realizaba esta práctica en las primeras 8 semanas de vida de las terneras. En 17 casos (47,2%) la práctica
era realizada por el operario encargado de los terneros ("guachero"). Diecinueve de los veterinarios encuestados (52,8%) manifestó una baja necesidad de administrar fármacos para mitigar el dolor durante estos procedimientos quirúrgicos menores. Veintinueve de los 36 veterinarios (80,6%) no utilizaba ningún tipo de fármaco para mitigar el dolor durante la maniobra, principalmente debido al tiempo que demanda (38,5%; 15/39) y los costos excesivos (25,6%; 10/39). Los 7 restantes (19,4%) aplicaba antiinflamatorios no esteroideos (42,8%; 3/7), anestésicos
locales (28,6%; 2/7), o la combinación de ambos (28,6%; 2/7), principalmente con motivo de respetar el bienestar de los animales (47,1%; 8/17), y lograr un manejo más dócil y seguro (29,4%; 5/17). Crecientemente el mercado y los consumidores exigen que se tomen medidas con respecto al bienestar animal (BA). Países de la Unión Europea y Norteamérica han adoptado estas medidas, por lo que las demandas no tardarán en difundirse globalmente a otros países exportadores de productos animales. -----------------------------------------------------------------
SUMMARY.- Disbudding/dehorning is a widespread practice that facilitates handling of dairy cattle, although it causes stress and pain. To
assess whether the application of pain management procedures (analgesia/anesthesia) is a common practice among veterinary practitioners from Uruguay and Argentina, an online questionnaire was distributed by e-mail to 120 veterinarians. Responses were obtained from 38 veterinarians, 94,7% (36/38) of whom either performed or indicated disbudding/dehorning in their dairy herds. Of these veterinarians, 75% (27/36) performed
this practice on calves ≤8 weeks of age. In 47,2% (17/36) of cases, the procedure was performed by the calf caregiver. Approximately 53% (19/36) of the surveyed veterinarians reported a low need to administer pain-relieving drugs during minor surgical procedures, and 80,6% (29/36) did not use any type of pain-relieving medication during the procedure, primarily due to the time required for the operation (38,5%; 15/39) and cost (25,6%; 10/39) considerations. The remaining 19,4% (7/36) administered non-steroidal anti-inflammatory drugs (42,8%; 3/7), local anesthetics (28,6%; 2/7), or a combination of both (28,7%; 2/7), out of concern for animal welfare (47,1%; 8/17) and to achieve docile and safe animal handling (29,4%; 5/17). The world market and consumers are demanding action on animal welfare. Countries of the European Union and North America have adopted pain management measures, which may soon be adopted to other exporting countries. MenosRESUMEN.- El desbotonamiento/descorne en los bovinos lecheros es una práctica muy difundida que facilita el manejo, aunque provoca
estrés y dolor. Para evaluar preliminarmente qué tan difundida es la aplicación de procedimientos de manejo del dolor (analgesia/anestesia) durante esta maniobra entre veterinarios asesores de tambos de Uruguay y Argentina, se realizó un cuestionario distribuido por correo electrónico a 120 veterinarios. Treinta y seis de los 38 veterinarios (94,7%) que respondieron a la encuesta realizaba este procedimiento en los tambos que asesoraba. De estos 36, el 75% (27) realizaba esta práctica en las primeras 8 semanas de vida de las terneras. En 17 casos (47,2%) la práctica
era realizada por el operario encargado de los terneros ("guachero"). Diecinueve de los veterinarios encuestados (52,8%) manifestó una baja necesidad de administrar fármacos para mitigar el dolor durante estos procedimientos quirúrgicos menores. Veintinueve de los 36 veterinarios (80,6%) no utilizaba ningún tipo de fármaco para mitigar el dolor durante la maniobra, principalmente debido al tiempo que demanda (38,5%; 15/39) y los costos excesivos (25,6%; 10/39). Los 7 restantes (19,4%) aplicaba antiinflamatorios no esteroideos (42,8%; 3/7), anestésicos
locales (28,6%; 2/7), o la combinación de ambos (28,6%; 2/7), principalmente con motivo de respetar el bienestar de los animales (47,1%; 8/17), y lograr un manejo más dócil y seguro (29,4%; 5/17). Crecientemente el mercado y los consumid... Presentar Todo |
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Palabras claves : |
Animal welfare; Cirugía menor; Dairy farming; Lechería bovina; Minor surgery; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Prácticas veterinarias; South America; Sudamérica; Veterinary practices. |
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Thesagro : |
BIENESTAR ANIMAL. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17305/1/Caffarena-et-al.-2018.-SMVU-v54-n210-04.pdf
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Marc : |
LEADER 04743naa a2200313 a 4500
001 1064270
005 2023-07-24
008 2018 bl uuuu u00u1 u #d
022 $a0376-4362 (impresa); 1688-4809 (en línea).
024 7 $a10.29155/VET.54.210.4$2DOI
100 1 $aCAFFARENA, D.
245 $aUso de prácticas de manejo del dolor durante el desbotonamiento y descorne de las terneras de tambo$bun estudio piloto en Uruguay y Argentina. [Use of pain management procedures during disbudding/dehorning of dairy heifers: a pilot study in Uruguay and Argentina.]$h[electronic resource]
260 $c2018
500 $aArticle history: Recibido 20 Diciembre 2017; Aceptado 29 Mayo 2018; Publicado 1 Noviembre 2018. -- Autor para correspondencia: dcaffarena@inia.org.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).
520 $aRESUMEN.- El desbotonamiento/descorne en los bovinos lecheros es una práctica muy difundida que facilita el manejo, aunque provoca estrés y dolor. Para evaluar preliminarmente qué tan difundida es la aplicación de procedimientos de manejo del dolor (analgesia/anestesia) durante esta maniobra entre veterinarios asesores de tambos de Uruguay y Argentina, se realizó un cuestionario distribuido por correo electrónico a 120 veterinarios. Treinta y seis de los 38 veterinarios (94,7%) que respondieron a la encuesta realizaba este procedimiento en los tambos que asesoraba. De estos 36, el 75% (27) realizaba esta práctica en las primeras 8 semanas de vida de las terneras. En 17 casos (47,2%) la práctica era realizada por el operario encargado de los terneros ("guachero"). Diecinueve de los veterinarios encuestados (52,8%) manifestó una baja necesidad de administrar fármacos para mitigar el dolor durante estos procedimientos quirúrgicos menores. Veintinueve de los 36 veterinarios (80,6%) no utilizaba ningún tipo de fármaco para mitigar el dolor durante la maniobra, principalmente debido al tiempo que demanda (38,5%; 15/39) y los costos excesivos (25,6%; 10/39). Los 7 restantes (19,4%) aplicaba antiinflamatorios no esteroideos (42,8%; 3/7), anestésicos locales (28,6%; 2/7), o la combinación de ambos (28,6%; 2/7), principalmente con motivo de respetar el bienestar de los animales (47,1%; 8/17), y lograr un manejo más dócil y seguro (29,4%; 5/17). Crecientemente el mercado y los consumidores exigen que se tomen medidas con respecto al bienestar animal (BA). Países de la Unión Europea y Norteamérica han adoptado estas medidas, por lo que las demandas no tardarán en difundirse globalmente a otros países exportadores de productos animales. ----------------------------------------------------------------- SUMMARY.- Disbudding/dehorning is a widespread practice that facilitates handling of dairy cattle, although it causes stress and pain. To assess whether the application of pain management procedures (analgesia/anesthesia) is a common practice among veterinary practitioners from Uruguay and Argentina, an online questionnaire was distributed by e-mail to 120 veterinarians. Responses were obtained from 38 veterinarians, 94,7% (36/38) of whom either performed or indicated disbudding/dehorning in their dairy herds. Of these veterinarians, 75% (27/36) performed this practice on calves ≤8 weeks of age. In 47,2% (17/36) of cases, the procedure was performed by the calf caregiver. Approximately 53% (19/36) of the surveyed veterinarians reported a low need to administer pain-relieving drugs during minor surgical procedures, and 80,6% (29/36) did not use any type of pain-relieving medication during the procedure, primarily due to the time required for the operation (38,5%; 15/39) and cost (25,6%; 10/39) considerations. The remaining 19,4% (7/36) administered non-steroidal anti-inflammatory drugs (42,8%; 3/7), local anesthetics (28,6%; 2/7), or a combination of both (28,7%; 2/7), out of concern for animal welfare (47,1%; 8/17) and to achieve docile and safe animal handling (29,4%; 5/17). The world market and consumers are demanding action on animal welfare. Countries of the European Union and North America have adopted pain management measures, which may soon be adopted to other exporting countries.
650 $aBIENESTAR ANIMAL
653 $aAnimal welfare
653 $aCirugía menor
653 $aDairy farming
653 $aLechería bovina
653 $aMinor surgery
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPrácticas veterinarias
653 $aSouth America
653 $aSudamérica
653 $aVeterinary practices
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tVeterinaria (Montevideo), 2018$gv. 54, no. 210, p. 22-26. -- OPEN ACCESS.
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